Our new Clear+ tissue clearing method is the sole strategy that delipidates samples without having improve in morphology and with minimal impact on structural integrity.
Megatome can be a vibrating microtome built to portion a wide range of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With higher blade vibrating frequency and minimized blade deflection, Megatome allows large-throughput tissue sectioning with uniform floor profile, together with negligible tissue destruction and information loss.
Antibodies may possibly just take months to diffuse by way of just a few millimeters of tissue, by using a steep labeling gradient from floor to core.
Running the CLI directly from a Gradle task is not really currently supported. A distribution must be established by using gradlew :j2d-cli:distZip to produce a zip file made up of anything required to operate.
SE makes use of a rotational electrical area to disperse extremely electromobile molecules (such as antibodies or surfactant micelles) all over a porous sample without the need of detrimental electrically billed constructions within the tissue. This enables two-four day clearing of intact organs,
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Megatome is actually a novel microtome which allows for prime-precision sectioning of a wide range of tissue samples – from organoids, to arrays of animal organs, to intact human Mind hemispheres – with nominal tissue damage and data loss.
eFLASH is usually a speedy tissue labeling technique that enables for uniform entire-organ staining in twenty rounds of labeling.
The SmartSPIM light-weight sheet microscope gives unparalleled resolution and acquisition velocity through intact tissue volumes, while its modular layout enables you to up grade and customize particular person components.
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Protect avoids the variability of hydrogel embedding and the information reduction from PFA preservation, shielding specimens for multiple rounds of processing.
Leverage the Apparent+ tissue clearing method, in addition to eFLASH and patented stochastic electrotransport systems, to promptly clear and label entire organs. Crucial highlights and capabilities involve:
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